well rabbit novus biologicals nbp1 48336 ldh a antibody blocking peptide elisa detection n a n a novus biologicals nbp1 48336pep ldh b elisa detection  (Novus Biologicals)

Journal: Neoplasia (New York, N.Y.)

Article Title: Metabolic and imaging phenotypes associated with RB1 and TP53 loss in prostate cancer

doi: 10.1016/j.neo.2025.101235

Figure Lengend Snippet: RB1 depletion leads to increased LDH activity. ELISA based LDH activity of (A) LuCaP167 organoids with or without ShRNA targeting RB1 and/or TP53 , and (B) LNCaP Crispr-mediated knockout (KO) of RB1 and/or TP53 . One-way ANOVA test with Dunnett’s multiple comparisons test was performed using Prism 9.0. p= * (0.05), ** (0.01), *** (0.001).

Article Snippet: Membranes were blocked in 5% milk (Cell Signaling: #9999) in TBST, followed by overnight antibody probing at 4°C°C with RB1 (CST, #9309S), TP53 (Abcam: #PAb240), AR (CST: #5153S), LDH (Novus: #NBP1-48336), KLK3 (CST: #5365), and Histone-H3 (CST: #4499S).

Techniques: Activity Assay, Enzyme-linked Immunosorbent Assay, shRNA, CRISPR, Knock-Out

Journal: Neoplasia (New York, N.Y.)

Article Title: Metabolic and imaging phenotypes associated with RB1 and TP53 loss in prostate cancer

doi: 10.1016/j.neo.2025.101235

Figure Lengend Snippet: 13 C-HPMRS shows depletion of RB1 alone or combined with TP53 depletion increases LDH flux in vivo . In vivo LDH activity in LuCaP 167 organoids PDX mouse xenografts with or without doxycycline-inducible shRNA knockdown of RB1 and/or TP53 calculated from time-resolved 13 C hyperpolarized non-localized spectra from the ratio of the area under the lactate and pyruvate signals. Data are represented as mean ± SD, ns = not significant Student t test was performed using Prism 9.0. p= * (0.05), ** (0.01), *** (0.001).

Article Snippet: Membranes were blocked in 5% milk (Cell Signaling: #9999) in TBST, followed by overnight antibody probing at 4°C°C with RB1 (CST, #9309S), TP53 (Abcam: #PAb240), AR (CST: #5153S), LDH (Novus: #NBP1-48336), KLK3 (CST: #5365), and Histone-H3 (CST: #4499S).

Techniques: In Vivo, Activity Assay, shRNA, Knockdown